Complete bypass of restriction systems for major staphylococcus aureus lineages

IR Monk; JJ Tree; BP Howden; TP Stinear; TJ Foster

Journal article

Complete bypass of restriction systems for major staphylococcus aureus lineages

IR Monk, JJ Tree, BP Howden, TP Stinear, TJ Foster

Mbio | Published : 2015

DOI: 10.1128/mBio.00308-15

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Abstract

Staphylococcus aureus is a prominent global nosocomial and community-acquired bacterial pathogen. A strong restriction barrier presents a major hurdle for the introduction of recombinant DNA into clinical isolates of S. aureus. Here, we describe the construction and characterization of the IMXXB series of Escherichia coli strains that mimic the type I adenine methylation profiles of S. aureus clonal complexes 1, 8, 30, and ST93. The IMXXB strains enable direct, high-efficiency transformation and streamlined genetic manipulation of major S. aureus lineages.

University of Melbourne Researchers

Ian Monk Author

Ben Howden Author

Tim Stinear Author

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Grants

Awarded by National Science Foundation

Funding Acknowledgements

We acknowledge financial support from Science Foundation Ireland (grant no. 08/IN.1/B1845) to TJF and National Health and Medical Research Council, Australia, fellowships to B.P.H. (APP1023526) and T.P.S. (APP1008549).